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All chromatographic separations, including HPLC work under the exact same essential basic principle; each and every compound interacts with other chemical species in a characteristic way. It's a particular method of column chromatography used in biochemistry and analysis to independent, estab

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Fractions are gathered from the bottom in the column, normally using exam tubes or other collection vessels. Each portion contains a mix of compounds which have comparable affinities for the stationary and mobile phases. Separation: Unique compounds inside the sample have various affinities to th